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Production of active human carbonic anhydrase II in E. coli
C Forsman1, G Behravan, A Osterman
1Department of Biochemistry, University of Umeå, Sweden.
Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry
|May 1, 1988
Abstract:
cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.