Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

13.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.6K
LTR Retrotransposons03:08

LTR Retrotransposons

19.9K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
19.9K
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

50.1K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
50.1K
Exon Recombination02:32

Exon Recombination

4.2K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
4.2K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.4K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.4K
Retroviruses02:33

Retroviruses

15.1K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
15.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

State-specific inhibition of NMDA receptors by memantine provides insight into NMDAR channel blocker tolerability.

Science advances·2026
Same author

Long-term effects of reovirus strain T3D on the myocardium.

Microbiology spectrum·2026
Same author

From defense to disease: the multifaceted role of lymphatics during infection.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Differential behavioral engagement of inhibitory interneuron subtypes in the zebra finch brain.

Neuron·2024
Same author

Correction: Development of ACE2 autoantibodies after SARS-CoV-2 infection.

PloS one·2024
Same author

The Mitochondrial Ubiquitin Ligase MARCHF5 Cooperates with MCL1 to Inhibit Apoptosis in KSHV-Transformed Primary Effusion Lymphoma Cell Lines.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Mar 2, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

9.5K

Reverse Genetics for Mammalian Orthoreovirus.

Johnasha D Stuart1, Matthew B Phillips1, Karl W Boehme2

  • 1Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, 72205, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2017
PubMed
Summary

This study presents a novel reverse genetics system for mammalian orthoreovirus. This system enables the creation of mutant viruses, advancing our understanding of viral diseases and vaccine development.

Keywords:
Double-stranded RNA virusPlasmid-based reverse geneticsRecombinant virusReovirusT7 RNA polymeraseViral reassortment

More Related Videos

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
13:48

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Published on: June 24, 2012

17.3K
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

18.1K

Related Experiment Videos

Last Updated: Mar 2, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

9.5K
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
13:48

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Published on: June 24, 2012

17.3K
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

18.1K

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Reverse genetics is crucial for understanding viral replication and pathogenesis.
  • Mutant viruses aid in developing vaccines and virus-based vectors.
  • Mammalian orthoreovirus is a double-stranded RNA virus with significant research interest.

Purpose of the Study:

  • To describe a novel reverse genetics system for mammalian orthoreovirus.
  • To enable the production and recovery of infectious viral particles from engineered plasmids.
  • To facilitate further research into orthoreovirus biology and applications.

Main Methods:

  • Construction of plasmids encoding the mammalian orthoreovirus genome.
  • Transfection of cells with these plasmids to initiate viral replication.
  • Recovery and characterization of infectious virus from transfected cells.

Main Results:

  • Successful establishment of a reverse genetics system for mammalian orthoreovirus.
  • Production of infectious virus from synthetic viral RNA genomes.
  • Demonstration of the system's utility for generating genetically modified orthoreoviruses.

Conclusions:

  • The developed reverse genetics system is a powerful tool for studying mammalian orthoreovirus.
  • This system will accelerate research into viral replication, pathogenesis, and the development of novel therapeutics and vaccines.
  • Enables precise genetic manipulation of this important dsRNA virus.