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Updated: Aug 15, 2026

An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay
Published on: January 26, 2019
BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV)
Christopher C Stobart1,2, Anne L Hotard1,2, Jia Meng1,2
1Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA.
Abstract:
Respiratory syncytial virus (RSV) is an enveloped, nonsegmented negative-strand RNA virus, which causes lower respiratory tract infections and is a leading cause of mortality in young infants. There is no available RSV vaccine and currently administered prophylactic antibodies are limited to high-risk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks.
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