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Human rotavirus infection enhances invasiveness of enterobacteria in MA-104 cells
1Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Norway.
Abstract:
To study the interaction between common pathogens causing infectious diarrhea in humans, MA 104 cell cultures were infected with human rotavirus and Salmonella typhimurium or Shigella flexneri or enteroinvasive Escherichia coli (EIEC) concomitantly. When MA-104 cells were preinfected with human rotavirus, invasiveness of S. typhimurium was significantly enhanced. The enhancement was evident after 48 h of virus preincubation. At this time virus specific antigens were demonstrated in the cell cultures. Also, the invasiveness of S. flexneri and EIEC was enhanced after virus preincubation, though not significantly before 72 h. When the virus preincubation time was prolonged to 96 h a further increase in invasive ability was observed. No cytopathogenic effect of the virus on the cells was demonstrated. Two control strains of non-enteropathogenic E. coli did not show any invasiveness in MA-104 cells pretreated with virus. The results indicate a specific interaction between rotavirus infected cells and facultatively intracellular enteropathogenic bacteria.
Insights
Human rotavirus infection enhances the invasiveness of pathogenic bacteria like Salmonella typhimurium in cell cultures. This specific interaction between rotavirus-infected cells and bacteria may impact infectious diarrhea pathogenesis.
Area of Science:
- Microbiology and Virology
- Cell Biology
- Infectious Diseases
Background:
- Infectious diarrhea is a significant global health concern caused by various pathogens.
- Understanding pathogen interactions is crucial for developing effective treatments and prevention strategies.
Purpose of the Study:
- To investigate the interaction between human rotavirus and common bacterial enteropathogens.
- To determine if rotavirus pre-infection affects the invasiveness of Salmonella typhimurium, Shigella flexneri, and enteroinvasive Escherichia coli (EIEC).
Main Methods:
- MA-104 cell cultures were co-infected with human rotavirus and bacterial pathogens.
- Cells were pre-infected with rotavirus for varying durations (48-96 hours) before bacterial challenge.
- Bacterial invasiveness was assessed in rotavirus-preinfected versus control cells.
Main Results:
- Pre-infection with human rotavirus significantly enhanced the invasiveness of Salmonella typhimurium.
- Invasiveness of Shigella flexneri and EIEC was also enhanced by rotavirus pre-infection, particularly after 72 hours.
- No cytopathic effects were observed in rotavirus-infected cells, and non-enteropathogenic E. coli showed no invasiveness.
Conclusions:
- Rotavirus infection creates a cellular environment that specifically promotes the invasiveness of certain enteropathogenic bacteria.
- This interaction suggests a complex interplay between viral and bacterial pathogens in the context of infectious diarrhea.
- Further research is warranted to elucidate the mechanisms underlying this enhanced bacterial invasiveness.