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Published on: April 15, 2021
Graphene Oxide Conjugated Magnetic Beads for RNA Extraction
Xuan-Hung Pham1, Ahruem Baek1, Tae Han Kim1
1Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, Republic of Korea.
Abstract:
A magnetic material that consists of silica-coated magnetic beads conjugated with graphene oxide (GO) was successfully prepared for facile ribonucleic acid (RNA) extraction. When the GO-modified magnetic beads were applied to separate the RNA from the lysed cell, the cellular RNAs were readily adsorbed to and readily desorbed from the surface of the GO-modified magnetic beads by urea. The amount of RNA extracted by the GO-modified magnetic beads was ≈170 % as much as those of the control extracted by a conventional phenol-based chaotropic solution. These results demonstrate that the facile method of RNA separation by using GO-modified magnetic beads as an adsorbent is an efficient and simple way to purify intact cellular RNAs and/or microRNA from cell lysates.
