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Updated: Mar 2, 2026
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
Differential expression of microRNA related to irritable bowel syndrome in a rabbit model
Qin Qin Yang1,2, Xiao Ping Xu1, Hong Shu Zhao1
1Experimental Animal Research Center, Zhejiang Chinese Medical University, Hangzhou, Zhejiang Province, China.
Objective:
This study aimed to evaluate the differential expressions of microRNAs (miRNAs) in white hair black eye (WHBE) rabbits of irritable bowel syndrome (IBS).
Methods:
WHBE and Japanese white (JW) rabbits were divided into the control and IBS groups. The IBS groups were exposed to moist heat, stress and low-dose laxatives. Their intestinal movement rate was measured. Blood samples were taken to detect serum 5-hydroxytryptamine (5-HT) and dopamine levels and colonic tissues were obtained to detect c-Fos expression by immunohistochemistry. Deep sequencing technology was used to obtain miRNA sequences in the intestinal tissues of WHBE and JW control groups. Expressions of 14 miRNAs were measured by real-time polymerase chain reaction in both the control and the IBS model groups.
Results:
Serum 5-HT and dopamine levels, intestinal movement rate and c-Fos expressions in the WHBE rabbits were significantly increased compared with the control group. MiR-29a-3p, miR-24-3p, miR-221-3p, let-7f-5p, let-7g-5p, let-7i-5p, miR-192-5p, miR-126-3p and miR-130b-3p expressions in WHBE IBS rabbits at day 14 were significantly higher than those in the control group while miR-324-3p and miR-132 were downregulated. MiR-29a-3p, let-7i-5p, miR-192-5p and miR-126-3p were significantly upregulated only in JW IBS rabbits at day 14 and miR-324-3p, miR-223-3p and miR-132 were significantly downregulated in JW IBS group. MiR-24-3p, miR-221-3p, let-7f-5p, miR-126-3p and miR-130b-3p expressions in WHBE IBS rabbits were higher than that in JW IBS rabbits.
Conclusions:
Twelve miRNAs were differentially expressed in IBS rabbits. Five are specific in WHBE IBS rabbits, suggesting that they play a role in increased sensitivity to IBS.
Insights
This study investigated microRNA (miRNA) expression in rabbits with irritable bowel syndrome (IBS). Specific miRNAs were found to be differentially expressed in WHBE rabbits, suggesting their role in IBS sensitivity.
Area of Science:
- Gastroenterology
- Molecular Biology
- Animal Models
Background:
- Irritable Bowel Syndrome (IBS) is a common gastrointestinal disorder.
- MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and are implicated in various diseases, including IBS.
- Understanding miRNA expression patterns can provide insights into IBS pathogenesis.
Purpose of the Study:
- To evaluate the differential expressions of microRNAs (miRNAs) in white hair black eye (WHBE) rabbits with experimentally induced irritable bowel syndrome (IBS).
- To compare miRNA expression profiles between WHBE and Japanese white (JW) rabbit models of IBS.
- To identify potential miRNA biomarkers associated with IBS in WHBE rabbits.
Main Methods:
- Irritable Bowel Syndrome (IBS) models were established in WHBE and Japanese white (JW) rabbits using moist heat, stress, and laxatives.
- Intestinal movement rate, serum 5-hydroxytryptamine (5-HT), dopamine levels, and colonic c-Fos expression were measured.
- Deep sequencing and real-time polymerase chain reaction (PCR) were employed to analyze miRNA expression in intestinal tissues.
Main Results:
- WHBE rabbits with IBS exhibited significantly increased serum 5-HT, dopamine, intestinal movement rate, and c-Fos expression compared to controls.
- Twelve miRNAs showed differential expression in IBS rabbits.
- Five specific miRNAs (miR-24-3p, miR-221-3p, let-7f-5p, miR-126-3p, miR-130b-3p) were upregulated in WHBE IBS rabbits and showed higher expression compared to JW IBS rabbits.
Conclusions:
- Differential expression of twelve miRNAs was observed in IBS rabbit models.
- Five specific miRNAs in WHBE IBS rabbits suggest a role in heightened IBS sensitivity.
- These findings highlight potential miRNA-based mechanisms contributing to IBS pathophysiology.
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