Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

931
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
931

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Critique of "The impact of foot reflexology on postoperative pain and fear in children following circumcision: A randomized controlled trial".

Journal of pediatric urology·2025
Same author

Comments on Fendereski et al., Comparing Penile Problems in Circumcised vs. Uncircumcised Boys: Insights From a Large Commercial Claims Database With a Focus on Provider Type Performing Circumcision.

Journal of pediatric surgery·2024
Same author

Comments by opponents on the British Medical Association's guidance on non-therapeutic male circumcision of children seem one-sided and may undermine public health.

World journal of clinical pediatrics·2024
Same author

Letter to Editor regarding: Referrals from primary care with foreskin symptoms: Is there really room for improvement?

Journal of pediatric surgery·2023
Same author

Light and Scanning Electron Microscopy of Red Blood Cells From Humans and Animal Species Providing Insights into Molecular Cell Biology.

Frontiers in physiology·2022
Same author

Critical evaluation of contrasting evidence on whether male circumcision has adverse psychological effects: A systematic review.

Journal of evidence-based medicine·2022

Related Experiment Video

Updated: Mar 2, 2026

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

1.3K

Biological applications of second harmonic imaging.

Guy Cox1

  • 1Australian Centre for Microscopy and Microanalysis, University of Sydney, NSW, 2006, Australia. Guy.Cox@sydney.edu.au.

Biophysical Reviews
|May 17, 2017
PubMed
Summary

Second Harmonic Generation (SHG) microscopy, developed in 1974, now excels in biological imaging of collagen, myosin, and polysaccharides. Recent advances also include SHG dyes for high-speed neurobiology applications.

Keywords:
CollagenMyosinPolysaccharidesSHGSHG dyesSecond Harmonic Microscopy

More Related Videos

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

668
Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

Published on: January 6, 2018

8.3K

Related Experiment Videos

Last Updated: Mar 2, 2026

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

1.3K
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

668
Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

Published on: January 6, 2018

8.3K

Area of Science:

  • Biomedical optics
  • Microscopy techniques
  • Molecular imaging

Background:

  • Second Harmonic Generation (SHG) microscopy, originating in 1974, has seen significant biological application development over the last decade.
  • SHG microscopy is instrumental in visualizing non-fluorescent biological structures like collagen.

Purpose of the Study:

  • To review the advancements and current applications of Second Harmonic Generation (SHG) microscopy in biological sciences.
  • To highlight the expanding utility of SHG in imaging diverse biomolecules and its emerging role in neurobiology.

Main Methods:

  • Review of existing literature on SHG microscopy applications.
  • Analysis of SHG microscopy's use in imaging collagen, myosin, and polysaccharides.
  • Examination of SHG dyes for membrane potential sensing in neurobiology.

Main Results:

  • SHG microscopy is now a standard technique for collagen imaging across various biological fields.
  • Emerging applications include the imaging of myosin and certain polysaccharides.
  • SHG dyes offer high-speed membrane potential detection, finding utility in neurobiological research.

Conclusions:

  • SHG microscopy has evolved from a foundational technique to a versatile tool in modern biological imaging.
  • The development of SHG dyes represents a significant advancement for high-speed cellular activity monitoring.
  • Continued research promises further expansion of SHG applications in life sciences.