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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
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Rhesus Macaque iPSC Generation and Maintenance.

Ravi Chandra Yada1, So Gun Hong1, Yongshun Lin2

  • 1Hematology Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health, Bethesda, Maryland.

Current Protocols in Stem Cell Biology
|May 17, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a method to create Rhesus induced pluripotent stem cells (RhiPSCs) using an excisable lentiviral vector. This technique allows for transgene removal, aiding in the preclinical assessment of cell therapies.

Keywords:
induced pluripotent stem cells (iPSCs)non-human primatereprogrammingrhesus macaque

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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Primate models

Background:

  • Rhesus macaques are valuable preclinical models due to physiological and phylogenetic similarity to humans.
  • Induced pluripotent stem cells (iPSCs) hold promise for cell therapies.
  • Efficient and safe methods for generating patient-specific cells are crucial.

Purpose of the Study:

  • To describe a method for generating Rhesus induced pluripotent stem cells (RhiPSCs).
  • To detail the use of an excisable STEMCCA vector for reprogramming.
  • To outline strategies for maintaining pluripotency during RhiPSC culture.

Main Methods:

  • Reprogramming of rhesus fibroblasts and bone marrow stromal cells (BMSCs) using a polycistronic lentiviral STEMCCA vector.
  • Transient expression of Cre recombinase to excise the reprogramming transgenes.
  • Culture conditions optimized for maintaining pluripotency during RhiPSC passaging.

Main Results:

  • Successful generation of RhiPSCs from multiple cell types.
  • Demonstration of transgene excision via Cre expression, leaving a genetic tag.
  • Establishment of protocols for stable RhiPSC culture and passaging.

Conclusions:

  • The described method provides a robust platform for generating RhiPSCs for preclinical research.
  • The excisable vector system facilitates the removal of reprogramming factors, enhancing safety for therapeutic applications.
  • This approach supports the use of RhiPSCs in advancing cell-based therapies and disease modeling.