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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Mar 2, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Constructing a Robust Fluorescent DNA-Stabilized Silver Nanocluster Probe Module by Attaching a Duplex Moiety.

Ruoyun Lin¹, Guangyu Tao¹, Yang Chen¹

¹Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, P. R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|May 17, 2017

Summary

Researchers developed robust fluorescent DNA-silver nanocluster (DNA-Ag NCs) probes by adding a duplex structure. This modification significantly enhances fluorescence, overcoming limitations of existing DNA-Ag NCs for broader applications.

Keywords:

fluorescence enhancement fluorescent DNA-Ag nanoclusters fluorescent probes nanoparticles nanospecies transformation

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Published on: August 26, 2009

Area of Science:

Nanotechnology
Biochemistry
Materials Science

Background:

Fluorescent DNA-templated silver nanoclusters (DNA-Ag NCs) are valuable luminescent probes.
Their fluorescence is highly sensitive to DNA template modifications, limiting applications.

Purpose of the Study:

To develop a robust fluorescent DNA-Ag NCs probe module with enhanced stability and fluorescence.
To overcome the limitations of current DNA-Ag NCs sensitivity to template changes.

Main Methods:

Constructed a DNA-Ag NCs probe module by attaching a duplex moiety to the nanocluster-bearing DNA sequence.
Investigated the effect of duplex hybridization on fluorescence intensity.
Analyzed the influence of adenine and thymine in the linker sequence on fluorescence.

Main Results:

The attachment of a duplex moiety significantly enhanced fluorescence intensity by up to 90-fold.
Duplex formation induced a transformation from a non-fluorescent to a fluorescent state.
Adenine in the linker enhanced fluorescence, while thymine caused quenching.

Conclusions:

The developed strategy creates robust fluorescent DNA-Ag NCs probes with significantly improved fluorescence.
This design diversifies experimental possibilities for DNA-Ag NCs applications.
The findings address current limitations in DNA-Ag NCs stability and sensitivity.