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Updated: Mar 2, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
Immunolocalization of chromosome-associated proteins in plants - principles and applications
Cristina Maria Pinto de Paula1, Vânia Helena Techio2
1Department of Biology, Federal University of Lavras, Lavras, Zip code 372000-000, Minas Gerais State, Brazil.
Abstract:
The use of the immunolocalization technique combined with cytogenetic and epigenetic studies is an indispensable tool and has contributed significantly to the analysis of the structure and function of chromosomes, since it can provide information about the spatial or temporal distribution of a given protein in the nucleus and chromosomes. Several chromosome-associated proteins in plant cells have already been identified by immunolocalization, such as histone and non-histone proteins and cell division-related protein (mitosis and meiosis). The principle of the immunolocalization technique in plants basically involves fixation and permeabilization of cells, the use of monoclonal or polyclonal antibodies attached to a signaling molecule, usually a fluorochrome and detection of the target molecule by using an epifluorescence microscope.
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