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Structural changes in DNA of human breast carcinoma
M Mackiewicz1, B Choina, A Kadlof
1Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
Neoplasma
|January 1, 1988
Abstract:
Rearrangement and loss of genetic material in DNA of human ductal breast carcinoma was analyzed by restriction enzyme digestion, agarose gel electrophoresis and Southern blot hybridization using 32P labeled c-Ha-ras-1 and multilocus, minisatellite 15.1.11.4 as a probe. In several cases an allelic loss of c-Ha-ras-1 locus in heterozygous patients were noticed. Rearrangement of the genetic material in neoplastic tissues was also observed after hybridization with a minisatellite probe. These results provide evidence that loss and redistribution of the genetic material does occur in human ductal breast carcinoma.