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Malassezia Yeast and Cytokine Gene Polymorphism in Atopic Dermatitis

Charu Jain1, Shukla Das2, V G Ramachandran2

  • 1Senior Resident, Department of Microbiology, UCMS and GTB Hospital, Delhi, India.

Journal of Clinical and Diagnostic Research : JCDR
|May 18, 2017
PubMed
Abstract

Insights

This study found a higher isolation rate of Malassezia yeast in Atopic Dermatitis (AD) patients. Specific cytokine gene polymorphisms in IL10 and IFN-γ were significantly associated with increased susceptibility to Malassezia infection in AD.

Area of Science:

  • Immunodermatology
  • Medical Mycology
  • Human Genetics

Background:

  • Atopic Dermatitis (AD) is a chronic inflammatory skin condition.
  • Malassezia yeast is implicated in exacerbating AD symptoms due to its immunomodulatory properties and interaction with a compromised skin barrier.
  • Genetic predisposition, specifically Single Nucleotide Polymorphisms (SNPs) in cytokine genes, may influence host susceptibility to Malassezia infection in AD.

Purpose of the Study:

  • To isolate Malassezia yeast from patients diagnosed with Atopic Dermatitis.
  • To investigate the association between cytokine gene polymorphism (IL10 and IFN-γ) and host susceptibility to Malassezia infection in AD patients compared to healthy controls.

Main Methods:

  • Prospective observational study involving 38 AD patients and 38 controls.
  • Isolation and identification of Malassezia yeast from skin scrapings using fungal culture.
  • Genotyping of SNPs in IL10 and IFN-γ genes using Amplification Refractory Mutations System-Polymerase Chain Reaction (ARMS-PCR).

Main Results:

  • Malassezia yeast was isolated from 63.1% of AD patients and 52.6% of controls.
  • Significant differences in allele and genotype distributions were observed for IL10-819/592C/T and IFN-γ+874A/T gene polymorphisms in the AD group.
  • The study identified a correlation between specific cytokine gene polymorphisms and increased susceptibility to Malassezia infection.

Conclusions:

  • The higher isolation rate of Malassezia in AD patients underscores its role in the condition.
  • Specific cytokine gene polymorphisms are significantly associated with host susceptibility to Malassezia infection in Atopic Dermatitis.
  • Understanding these genetic factors can provide insights into the pathogenesis of AD.
Keywords:
GlobosaInterferonInterleukinSingle nucleotide polymorphismSympodialis

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