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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
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Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section.

Sneha S Chavan1, Savithri Ravindra2, Msn Prasad3

  • 1Postgraduate Student, Department of Pathology, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India.

Journal of Clinical and Diagnostic Research : JCDR
|May 18, 2017
PubMed
Summary

Tissue Microarray (TMA) analysis of Estrogen Receptor (ER), Progesterone Receptor (PR), and Her2/neu in breast cancer shows good concordance with traditional whole sections. This makes TMA a reliable, cost-effective method for biomarker evaluation.

Keywords:
Breast carcinomaEstrogen receptorHer2/Neu

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A Next-generation Tissue Microarray ngTMA Protocol for Biomarker Studies
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Area of Science:

  • Oncology
  • Pathology
  • Biomarker Analysis

Background:

  • Estrogen Receptor (ER), Progesterone Receptor (PR), and Her2/neu are critical biomarkers in breast carcinoma.
  • Traditionally, these markers are assessed using Immunohistochemistry (IHC) on whole tissue sections.
  • Tissue Microarray (TMA) offers an alternative method for analyzing multiple tissue samples efficiently.

Purpose of the Study:

  • To compare the accuracy of ER, PR, and Her2/neu assessment on TMA sections versus whole tissue sections.
  • To determine the concordance rate between biomarker results obtained from TMA and whole sections.

Main Methods:

  • A TMA block was created using 2 mm cores from 53 breast carcinoma cases.
  • Immunostaining for ER, PR, and Her2/neu was performed on both TMA and whole tissue sections.
  • Statistical analysis, including kappa coefficient, was used to compare results.

Main Results:

  • The study found good concordance between whole sections and TMA sections for ER (kappa=0.671), PR (kappa=0.754), and Her2/neu (kappa=1.000).
  • The majority of tumors (71.7%) were ER/PR positive and Her2 negative, with 17% being triple-negative.
  • Results were statistically significant, indicating reliability of the TMA method.

Conclusions:

  • Immunostaining for ER, PR, and Her2/neu on single 2 mm TMA cores is comparable to conventional whole section analysis.
  • TMA is a dependable technique for evaluating these essential breast cancer biomarkers.
  • TMA presents a time- and cost-effective advantage for biomarker assessment in breast carcinoma.