Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reproducible 3D Glioblastoma Migration Assay with Magnetic Nanoparticle Mediated Spheroid Localization Under Hypoxic Conditions.

Journal of visualized experiments : JoVE·2026
Same author

Metabolic reprogramming of human macrophages drives the formation of hybrid M1/M2 pro-regenerative extracellular vesicles.

Biomaterials·2026
Same author

Evaluation of tumor clonality with chemical carcinogenesis in a mouse model of visualized X chromosome inactivation.

Journal of toxicologic pathology·2026
Same author

Laser patterning of ECM-derived biomaterials to direct degradation, site-specific resorption, controlled vascularization and functional repair of large nerve defects.

Acta biomaterialia·2026
Same author

A novel workflow for multi-modal imaging of musculoskeletal tissues.

Journal of anatomy·2025
Same author

Correction: Selenium nanoparticle-functionalized injectable chitosan/collagen hydrogels as a novel therapeutic strategy to enhance stem cell osteoblastic differentiation for bone regeneration.

Journal of materials chemistry. B·2024

Related Experiment Video

Updated: Mar 2, 2026

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
08:24

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

Published on: October 8, 2010

18.3K

A method for isolating cortical interneurons sharing the same birthdays for gene expression studies.

Hui Xuan Ng1, Ean Phing Lee2, Brenton L Cavanagh3

  • 1Florey Institute of Neuroscience and Mental Health, Parkville, VIC, Australia; University of Melbourne, Parkville, VIC, Australia.

Experimental Neurology
|May 18, 2017
PubMed
Summary

Researchers developed a new method to isolate developing interneurons based on their birthdate and neurotransmitter identity. This technique enables gene expression analysis of specific neuronal populations, advancing the study of brain molecular diversity.

Keywords:
CortexEdUFACSGFPInterneuronRNAqPCR

More Related Videos

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
10:24

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

11.0K
Subtype-selective Electroporation of Cortical Interneurons
06:42

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

9.2K

Related Experiment Videos

Last Updated: Mar 2, 2026

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
08:24

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

Published on: October 8, 2010

18.3K
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
10:24

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Published on: December 3, 2017

11.0K
Subtype-selective Electroporation of Cortical Interneurons
06:42

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

9.2K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Cortical neurons, including pyramidal cells and interneurons, exhibit significant molecular diversity.
  • Studying molecular diversity is challenging due to the difficulty in isolating neuronal populations born at different developmental times.
  • Developing interneurons can be identified by Glutamic Acid Decarboxylase-67 (GAD67) expression.

Purpose of the Study:

  • To develop a method for isolating specific populations of developing interneurons for gene expression analysis.
  • To enable the study of molecular diversity in coetaneous (born at the same time) interneurons.
  • To overcome limitations in current methods for analyzing gene expression in distinct neuronal birth cohorts.

Main Methods:

  • Utilized GAD67-knockin GFP transgenic mice to identify interneurons.
  • Employed 5-ethynyl-2'-deoxyuridine (EdU) for neuronal birthdating.
  • Developed a fluorescent-activated cell sorting (FACS) strategy to simultaneously detect GFP and EdU signals.
  • Isolated coetaneous interneurons based on neurotransmitter identity and developmental timing.

Main Results:

  • Successfully isolated migrating interneurons using FACS based on combined GAD67 expression and EdU labeling.
  • Achieved simultaneous detection of GFP and EdU signals with minimal RNA integrity loss.
  • Confirmed satisfactory RNA quality for gene expression analysis via quantitative polymerase chain reaction (qPCR) of the Gad67 transcript.

Conclusions:

  • The developed FACS method effectively isolates coetaneous interneurons.
  • This technique facilitates RNA extraction and gene expression analysis of specific neuronal populations.
  • The method advances the investigation of molecular diversity within developing cortical interneurons.