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Two-step phase shifting differential-recording digital holographic microscopy
Jun-He Han1, Ruo-Ping Li2, Jun-Hui Liu2
1School of Physics and Electronics, Henan University, Kaifeng, 475004, China. junhh@henu.edu.cn.
Scientific Reports
|May 18, 2017
Summary
We developed two-step phase-shifting differential-recording digital holographic microscopy (TPD-DH) for faster phase imaging. This microscopy technique halves measurement time while maintaining accuracy for transparent samples.
Area of Science:
- Optics and Photonics
- Microscopy
- Digital Holography
Background:
- Phase imaging is crucial for analyzing transparent microscopic structures.
- Conventional methods like four-step phase-shifting interferometry (FS-PSI) can be time-consuming.
- Accurate reconstruction of amplitude and phase is essential for detailed analysis.
Purpose of the Study:
- To introduce a novel two-step phase-shifting differential-recording digital holographic microscopy (TPD-DH) technique.
- To enable faster and efficient phase imaging of microscopic transparent elements.
- To reduce measurement time without compromising accuracy.
Main Methods:
- Utilized two CCD cameras to record interferograms at distinct defocusing distances.
- Employed an all-optics phase shifting unit for 0 and π phase shifts.
- Developed a novel algorithm for reconstructing object wave amplitude and phase distributions.
Main Results:
- The TPD-DH method achieves the same spectrum bandwidth and measurement accuracy as FS-PSI.
- Successfully reconstructed both amplitude and phase distributions of transparent objects.
- Reduced the overall measurement time by 50% compared to conventional methods.
Conclusions:
- TPD-DH offers a significantly faster alternative for phase imaging in microscopy.
- The technique provides high accuracy and bandwidth comparable to established methods.
- This advancement is beneficial for studying dynamic transparent microscopic phenomena.
