Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination of Crystal Structures01:29

Determination of Crystal Structures

12
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
12
X-ray Crystallography02:18

X-ray Crystallography

26.5K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.5K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.0K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solvent- and heat-induced polymorphic transformation with single-crystal integrity in Cu(II) paddle wheel metal complexes.

Chemical communications (Cambridge, England)·2026
Same author

Xerogel-Derived Bis-Imidazole Semiconductors: Chain-Length-Controlled Gelation and Polycrystalline Blue OLED Emission.

ACS applied materials & interfaces·2026
Same author

Acta Crystallographica Section B welcomes three new Co-editors.

Acta crystallographica Section B, Structural science, crystal engineering and materials·2026
Same author

Copper Complexes of the Biginelli-Type Ligand: Effect of Solvents and Halide Ions on Crystal Structure.

Inorganic chemistry·2025
Same author

Examining the Role of Conformational Rigidity to Antibacterial Activity via Side-Chain Crosslinking of Amphiphilic Aromatic Oligoamides.

Chemistry, an Asian journal·2025
Same author

Acid-Responsive Two-photon Absorption Switch via Cocrystal-to-Salt-to-Cocrystal Conversion.

Angewandte Chemie (International ed. in English)·2025
Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026
Same journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026
Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026
Same journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026
See all related articles

Related Experiment Video

Updated: Mar 2, 2026

Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

7.2K

Acemetacin cocrystal structures by powder X-ray diffraction.

Geetha Bolla1, Vladimir Chernyshev2,3, Ashwini Nangia1,4

  • 1School of Chemistry, University of Hyderabad, Prof. C. R. Rao Road, Central University PO, Hyderabad 500 046, India.

Iucrj
|May 18, 2017
PubMed
Summary
This summary is machine-generated.

This study details the creation and structural analysis of acemetacin cocrystals using melt crystallization and X-ray diffraction. The research elucidates molecular packing and hydrogen bonding, revealing insights into pharmaceutical cocrystal design.

Keywords:
co-crystalscrystal engineeringmolecular crystals

More Related Videos

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects
09:16

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects

Published on: June 8, 2016

16.8K
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.9K

Related Experiment Videos

Last Updated: Mar 2, 2026

Microcrystal Electron Diffraction of Small Molecules
09:48

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

7.2K
X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects
09:16

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects

Published on: June 8, 2016

16.8K
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.9K

Area of Science:

  • Solid-state chemistry
  • Pharmaceutical science
  • Crystallography

Background:

  • Acemetacin (ACM) is a non-steroidal anti-inflammatory drug with limited solubility.
  • Cocrystallization is a strategy to improve the physicochemical properties of active pharmaceutical ingredients.
  • Understanding the solid-state structure of cocrystals is crucial for drug development.

Purpose of the Study:

  • To prepare and characterize cocrystals of acemetacin (ACM) with various coformers.
  • To determine the crystal structures of ACM cocrystals using high-resolution powder X-ray diffraction.
  • To investigate the molecular packing, hydrogen bonding, and conformational preferences within these cocrystals.

Main Methods:

  • Melt crystallization for cocrystal preparation.
  • High-resolution powder X-ray diffraction for structure determination.
  • Structure determination from powder data (SDPD) technique.
  • Molecular electrostatic potential (MEP) surface calculations.
  • Hirshfeld surface analysis.

Main Results:

  • Cocrystals of ACM with nicotinamide (NAM), p-aminobenzoic acid (PABA), valerolactam (VLM), and 2-pyridone (2HP) were successfully prepared.
  • The carboxylic acid group of ACM consistently formed acid-amide dimer synthons with various amides.
  • Two distinct conformations (Type I and Type II) of the ACM molecule were observed, influenced by the coformer and crystal form.
  • Hirshfeld surface analysis indicated significant contributions from O⋯H, N⋯H, Cl⋯H, and C⋯H interactions.

Conclusions:

  • Melt crystallization is an effective method for preparing acemetacin cocrystals.
  • The crystal structures reveal specific hydrogen bonding patterns and conformational variations of ACM.
  • These findings provide valuable insights for the rational design of novel acemetacin-based pharmaceutical cocrystals with improved properties.