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Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor Platform.

Myeong-Sub Song1, Simranjeet Singh Sekhon2, Woo-Ri Shin3

  • 1School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, Korea. smst04@nate.com.

Molecules (Basel, Switzerland)
|May 18, 2017
PubMed
Summary

Researchers developed aptamer-based biosensors to detect Shigella sonnei, a bacterium causing shigellosis. This novel Whole-Bacteria SELEX (WB-SELEX) method identified highly specific aptamers, enabling rapid and accurate pathogen detection in food samples.

Keywords:
DNA aptameraptamer-based fluorescent biosensorshigellosiswhole cell-SELEX

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensor Technology

Background:

  • Shigella sonnei is a significant cause of bacterial dysentery (shigellosis), necessitating rapid and accurate detection methods.
  • Current detection methods can be time-consuming or lack specificity, posing challenges for timely diagnosis and public health interventions.

Purpose of the Study:

  • To develop and validate a novel aptamer-based biosensor for the specific detection and discrimination of Shigella sonnei.
  • To utilize the Whole-Bacteria SELEX (WB-SELEX) strategy for isolating high-affinity aptamers against S. sonnei.

Main Methods:

  • Whole-Bacteria SELEX (WB-SELEX) was employed to isolate aptamers with high affinity and specificity for S. sonnei.
  • Selected aptamers (SS-3 and SS-4) were characterized for binding affinity and specificity using real-time PCR.
  • An aptamer-based fluorescent biosensor was constructed using a sandwich assay format with SS-3 and SS-4 aptamers for detection.

Main Results:

  • The SS-3 and SS-4 aptamers exhibited high binding affinity (K values of 39.32 ± 5.02 nM and 15.89 ± 1.77 nM, respectively) and specificity for S. sonnei.
  • The developed fluorescent biosensor successfully detected and discriminated S. sonnei from other enteric bacteria, including E. coli, Salmonella typhimurium, and other Shigella species.
  • The biosensor platform demonstrated feasibility for detecting S. sonnei in various food matrices.

Conclusions:

  • Aptamer-based fluorescent biosensors offer a promising platform for the sensitive and specific detection of Shigella sonnei.
  • The WB-SELEX approach is effective for generating aptamers against whole bacterial cells.
  • This technology has the potential for portable and rapid diagnostic tools for shigellosis and food safety applications.