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A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells
Ina Ersing1, Luis Nobre2, Liang Wei Wang3
1Division of Infectious Disease, Department of Medicine, Brigham & Women's Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA; Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
Cell Reports
|May 18, 2017
Summary
Epstein-Barr virus (EBV) replication involves significant host cell changes. This study maps viral and host proteins during EBV lytic replication, revealing immune pathway remodeling and conserved interactions.
Area of Science:
- Virology
- Immunology
- Proteomics
Background:
- Epstein-Barr virus (EBV) is linked to various human diseases.
- Understanding EBV's lytic replication and host interactions is crucial for disease control.
Purpose of the Study:
- To quantitatively analyze temporal changes in host and EBV proteins during lytic replication.
- To gain insights into virus-host interactions during EBV infection.
Main Methods:
- Systematic quantitative proteomic analysis of host and EBV proteins.
- Utilized conditional Burkitt lymphoma models for EBV types I and II infection.
- Quantified over 8,000 cellular and 69 EBV proteins, including plasma membrane proteins.
Main Results:
- Revealed EBV-induced remodeling of cell cycle and immune pathways.
- Observed upregulation of the complement cascade and proteasomal degradation of the B cell receptor complex.
- Identified conserved host factors targeted by multiple herpesviruses through cross-comparison.
Conclusions:
- Provides a temporal view of the lytic B cell proteome and EBV virome.
- Highlights conserved virus-host interactions during EBV lytic replication.
- Offers a valuable resource for EBV research and therapeutic development.
