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Updated: Mar 2, 2026

Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein
Published on: April 26, 2024
Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification
Christine M Ring1, Emil S Iqbal, David E Hacker
1Virginia Commonwealth University, Department of Chemistry, 1001 West Main Street, Richmond, Virginia 23284-2006, USA. tacropp@vcu.edu.
Abstract:
Due to the lowered pKa of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures.

