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Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields TTFields
Published on: May 4, 2017
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Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields (TTFields)
Yaara Porat1, Moshe Giladi2, Rosa S Schneiderman1
1Preclinical Research Department, Novocure Ltd., Haifa, Israel.
Journal of Visualized Experiments : Jove
|May 19, 2017
Summary
Tumor Treating Fields (TTFields) effectively treat cancer using alternating electric fields. The optimal frequency for TTFields in ovarian and glioma cells was found to be 200 kHz, enhancing treatment outcomes.
Area of Science:
- Oncology
- Biophysics
- Biomedical Engineering
Background:
- Tumor Treating Fields (TTFields) represent a non-invasive cancer treatment using low-intensity electric fields.
- Understanding optimal parameters for TTFields in vitro is crucial for refining treatment efficacy.
Purpose of the Study:
- To determine the optimal frequency for Tumor Treating Fields (TTFields) in cancer cell lines.
- To evaluate the impact of TTFields frequency on cell proliferation and clonogenic survival.
Main Methods:
- Utilized a TTFields in vitro application system with ceramic Petri dishes (dielectric constant > 5,000).
- Applied TTFields at various frequencies and intensities to ovarian and glioma cell lines cultured on coverslips.
- Assessed treatment outcomes using cell counts and clonogenic assays.
Main Results:
- The optimal frequency for TTFields demonstrated significant effects on both cell counts and clonogenic assays.
- A frequency of 200 kHz was identified as optimal for both ovarian and glioma cancer cells.
Conclusions:
- 200 kHz is the optimal frequency for TTFields application in vitro for ovarian and glioma cells.
- This finding supports the optimization of TTFields therapy for improved cancer treatment outcomes.
