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Dissection of Larval Zebrafish Gonadal Tissue
Published on: April 26, 2017
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Dissection of Larval Zebrafish Gonadal Tissue
Xinjian Wang1, Sijie Chen1, Wei Zhang1
1Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University.
Journal of Visualized Experiments : Jove
|May 19, 2017
Summary
Domesticated zebrafish use polygenic sex determination, unlike wild types. This study details a method to isolate gonadal tissue from zebrafish larvae for studying sex development genes.
Area of Science:
- Developmental Biology
- Genetics
- Aquaculture
Background:
- Wild zebrafish exhibit ZZ/ZW sex determination, while domesticated strains have lost sex chromosomes.
- Domesticated zebrafish rely on a polygenic sex determination system involving multiple genes.
- The specific genes regulating gonad development in domesticated zebrafish are currently unknown.
Purpose of the Study:
- To establish a reliable procedure for isolating gonadal tissue from zebrafish larvae.
- To facilitate future research into the genetic basis of sex determination and gonad development in domesticated zebrafish.
Main Methods:
- A detailed protocol for isolating gonadal tissue from 17 and 25 days post fertilization (dpf) zebrafish larvae is presented.
- The isolated tissue can be used for morphological examination.
- The isolated tissue is suitable for gene expression profiling.
Main Results:
- A reproducible method for gonadal tissue isolation from specific larval stages was successfully developed.
- This procedure enables further investigation into the molecular mechanisms of sex development.
Conclusions:
- The presented method provides a crucial first step for dissecting the polygenic sex determination system in domesticated zebrafish.
- This technique will aid in identifying key genes involved in gonad development and sex identity.
