Application of a dot blot hybridization assay for the diagnosis of CMV infection or reactivation

Dot blot hybridization was performed for the detection of cytomegalovirus (CMV) genomes in urine samples. This assay was applied to the diagnosis of CMV infection in transplant patients, who were tested continuously after transplantation and the results were compared to the detection of early antigen (EA) in fibroblasts inoculated with urine specimens as well as to serological methods. It turned out that discrepancies between EA-detection and dot blot hybridization are partially caused by the different appearance and disappearance of the two parameters at different time points. In most cases the dot blot hybridization assay proved to be an earlier marker than EA-detection in the course of infection. In several patients, however, hybridization showed positive signals although there was no sign for a productive CMV infection.