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Related Experiment Video

Updated: Mar 2, 2026

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
06:28

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

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Operator-based variability of equine thromboelastography.

Kristen Thane1, Daniela Bedenice1, Ana Pacheco1,2

  • 1Department of Clinical Sciences, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA, 01536.

Journal of Veterinary Emergency and Critical Care (San Antonio, Tex. : 2001)
|May 19, 2017
PubMed
Summary

Equine thromboelastography (TEG) shows high reproducibility for clot strength (MA) and viscoelastic strength (G), but variability in clot formation time (K). This impacts early clot formation assessment in horses.

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Area of Science:

  • Veterinary Hematology
  • Coagulation Diagnostics
  • Thromboelastography Applications

Background:

  • Thromboelastography (TEG) is a valuable tool for assessing hemostasis in veterinary medicine.
  • Understanding the variability of TEG parameters is crucial for accurate clinical interpretation in horses.

Purpose of the Study:

  • To quantify intraoperator and interoperator variability in kaolin-activated equine TEG.
  • To identify TEG variables with the highest and lowest reproducibility in horses.

Main Methods:

  • Kaolin-activated TEG performed in duplicate on samples from 20 hospitalized horses.
  • Two operator pairs (A/B, A/C) assessed TEG variables: R, K, α, MA, and G.
  • Variability reported as coefficients of variation (CV); statistical analysis used paired t-test or Wilcoxon signed-rank test.
Keywords:
coagulationhorsesvariation

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Last Updated: Mar 2, 2026

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Main Results:

  • Intraoperator CVs ranged from 1.2% to 18%; interoperator CVs showed similar ranges.
  • Maximum Amplitude (MA) and Shear Strength (G) exhibited the lowest variability (1.2-7.3%).
  • Clot Formation Time (K) demonstrated the highest variability (13-23%); 70% of horses had values outside reference intervals.

Conclusions:

  • TEG measurements of MA and G are highly reproducible in horses, both within and between operators.
  • The early phase of clot formation (K) shows the most significant variability, impacting diagnostic accuracy.
  • Clinical coagulation status assessment was largely consistent between operators.