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Published on: June 9, 2011
Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014
Abstract:
A 4-year study (2011–2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were positive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) were positive for enteroviruses using cell culture methods. The highest detection rate was observed in the summer and autumn. In total, 185 enteroviruses were identified by using neutralization test. Echovirus 6 and 30 were the most common (41.7% and 37.5% respectively). The highest frequency of neurological infections (32.7%) occurred in children aged 5–9 years, mostly males (63.9%).
Insights
Enteroviruses are common causes of meningitis, particularly in children aged 5-9. Echovirus 6 and 30 were the most frequent culprits identified through RT-PCR and cell culture methods.
Area of Science:
- Virology
- Infectious Diseases
- Public Health
Background:
- Meningitis is a serious infection affecting the central nervous system.
- Enteroviruses are a significant cause of viral meningitis globally.
- Understanding enterovirus epidemiology is crucial for prevention and control.
Purpose of the Study:
- To investigate the prevalence and types of enteroviruses causing meningitis.
- To identify demographic and seasonal patterns of enterovirus infections.
- To compare detection rates using RT-PCR and cell culture.
Main Methods:
- Retrospective analysis of cerebrospinal fluid and clinical samples from 2011–2014.
- Detection of enteroviruses using reverse transcription-polymerase chain reaction (RT-PCR) and cell culture.
- Identification of enterovirus types via neutralization tests.
Main Results:
- Enteroviruses were detected in 67.8% of cerebrospinal fluid samples (RT-PCR) and 64.7% of clinical samples (cell culture).
- Echovirus 6 (41.7%) and Echovirus 30 (37.5%) were the most common types identified.
- Neurological infections were most frequent in males aged 5–9 years (32.7%), with peak detection in summer and autumn.
Conclusions:
- Enteroviruses are a major cause of meningitis, with Echovirus 6 and 30 being predominant.
- RT-PCR and cell culture are effective diagnostic methods for enterovirus detection.
- Children aged 5–9 years, particularly males, are most susceptible, with seasonal variations observed.
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