Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:29

The Extracellular Matrix

12.8K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.8K
The Extracellular Matrix01:42

The Extracellular Matrix

90.2K
Overview
90.2K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

5.7K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First-in-human phase I study of hypoxia-targeting <sup>64</sup>Cu-ATSM radioligand therapy in patients with malignant brain tumors (STAR-64).

ESMO open·2026
Same author

Reversal of left-sided colostomy utilizing single-port laparoscopy a multicenter European audit and overview of the literature.

Surgical endoscopy·2021
Same author

Functional Regulatory Mechanisms Underlying Bone Marrow Mesenchymal Stem Cell Senescence During Cell Passages.

Cell biochemistry and biophysics·2021
Same author

Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke.

Clinical and experimental immunology·2020
Same author

Oral environment and taste function of Japanese HIV-infected patients treated with antiretroviral therapy.

AIDS care·2019
Same author

Reparative bone-like tissue formation in the tooth of a systemic sclerosis patient.

International endodontic journal·2018

Related Experiment Video

Updated: Mar 2, 2026

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
04:14

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

Published on: June 19, 2019

5.8K

MSC/ECM Cellular Complexes Induce Periodontal Tissue Regeneration.

M Takewaki1, M Kajiya1, K Takeda1

  • 11 Department of Periodontal Medicine, Applied Life Sciences, Institute of Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan.

Journal of Dental Research
|May 19, 2017
PubMed
Summary

Transplanting cell clumps (C-MSCs) with self-produced matrix into periodontal defects promotes tissue regeneration. Osteogenic-differentiated C-MSCs accelerate alveolar bone repair, offering a novel regenerative therapy.

Keywords:
C-MSCMSCsartificial scaffold freecell therapy, tissue engineeringosteoinduction

More Related Videos

Isolation, Processing and Analysis of Murine Gingival Cells
09:47

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

20.0K
Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.8K

Related Experiment Videos

Last Updated: Mar 2, 2026

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
04:14

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

Published on: June 19, 2019

5.8K
Isolation, Processing and Analysis of Murine Gingival Cells
09:47

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

20.0K
Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

5.8K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Periodontology

Background:

  • Mesenchymal stem cells (MSCs) show promise for periodontal tissue regeneration.
  • Current implantation techniques require further refinement for predictability.
  • Novel scaffold-free approaches using cell-ECM complexes are emerging.

Purpose of the Study:

  • To evaluate the periodontal tissue regenerative potential of cell-matrix complexes (C-MSCs) in a canine model.
  • To assess the impact of osteogenic differentiation on C-MSC regenerative capacity.
  • To investigate scaffold-free C-MSC transplantation for periodontal defects.

Main Methods:

  • Mesenchymal stem cells (MSCs) were cultured to form cell-extracellular matrix (ECM) complexes (C-MSCs).
  • C-MSCs were cultured in growth or osteoinductive medium (OIM).
  • Periodontal tissue regeneration was assessed in a class III furcation defect model in beagle dogs.

Main Results:

  • C-MSCs, composed of cells and type I collagen, demonstrated osteogenic potential with OIM.
  • Both C-MSCs and OIM-treated C-MSCs induced periodontal tissue regeneration without scaffolds.
  • Osteogenic-differentiated C-MSCs significantly accelerated alveolar bone regeneration.

Conclusions:

  • Scaffold-free transplantation of C-MSCs is a viable strategy for periodontal regeneration.
  • Osteogenic differentiation enhances the bone regenerative capacity of C-MSCs.
  • C-MSC technology presents a novel and predictable approach for periodontal regenerative therapy.