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Updated: Mar 2, 2026
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
Published on: September 29, 2017
The influence of solvent formulations on thermosensitive hydroxybutyl chitosan hydrogel as a potential delivery
Zixian Bao1, Changqing Jiang2, Zhiguo Wang3
1College of Marine Life Science, Ocean University of China, Yushan Road, Qingdao 266003, Shandong Province, China.
Abstract:
Many cell delivery matrices have been developed due to the low transplantation efficiency of cell therapy. In the present study, thermosensitive hydroxybutyl chitosan (HBC) hydrogels were prepared with different formulations' solvent (Dulbecco's modified eagle's medium/phosphate buffered saline [DMEM/PBS], 0:100, 30:70, 50:50, 70:30, 100:0 [v/v]). Their gelation temperature was raised with DMEM ratio increase (from 9.5°C to 20.9°C). Pore sizes of HBC hydrogels treated with high ionic strength solutions became smaller. HUVECs cultured with HBC hydrogels exhibited proliferation in high DMEM ratio groups, and the optimal solvent formulation was DMEM/PBS 70:30 (v/v). Upon exposure to PBS, HUVECs encapsulated in HBC hydrogels could survive better than that when seeded on the surface of HBC hydrogels. These results demonstrated that HBC hydrogel could be a potential cell delivery matrix for cell therapy applications, especially when DMEM proportion in solvent formulations was higher than 50% (DMEM/PBS>50:50 [v/v]).
