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Updated: Mar 2, 2026

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
Published on: September 18, 2013
Lineage-dependent spatial and functional organization of the mammalian enteric nervous system
Reena Lasrado1, Werend Boesmans1,2, Jens Kleinjung1
1The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
None:
The enteric nervous system (ENS) is essential for digestive function and gut homeostasis. Here we show that the amorphous neuroglia networks of the mouse ENS are composed of overlapping clonal units founded by postmigratory neural crest-derived progenitors. The spatial configuration of ENS clones depends on proliferation-driven local interactions of ENS progenitors with lineally unrelated neuroectodermal cells, the ordered colonization of the serosa-mucosa axis by clonal descendants, and gut expansion. Single-cell transcriptomics and mutagenesis analysis delineated dynamic molecular states of ENS progenitors and identified RET as a regulator of neurogenic commitment. Clonally related enteric neurons exhibit synchronous activity in response to network stimulation. Thus, lineage relationships underpin the organization of the peripheral nervous system.
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