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Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for...

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.

UV–Vis Spectroscopy: Molecular Electronic Transitions

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In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...

Spectrophotometry: Introduction

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Related Experiment Video

Updated: Mar 2, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Choosing the optimal spectroscopic toolkit to understand protein function.

Michael A Hough¹

¹School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO7 6SR, U.K. mahough@essex.ac.uk.

Bioscience Reports

|May 20, 2017

Summary

This summary is machine-generated.

Spectroscopy is a powerful tool for studying protein properties and reactions. This editorial reviews key spectroscopic methods and their applications in biological research.

Keywords:

Combined methods EPR spectroscopy NMR spectroscopy Protein spectroscopy Time-resolved spectroscopy structural biology

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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

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Area of Science:

Biochemistry and Molecular Biology
Chemical Physics

Background:

Spectroscopy is a foundational technique in protein science, enabling the study of molecular properties and reaction mechanisms.
The evolution of spectroscopy has led to a diverse array of methods applicable to various biological systems and timescales.

Discussion:

This editorial provides an overview of essential spectroscopic techniques relevant to protein research.
Illustrative examples are presented to demonstrate the practical application of these methods.

Key Insights:

Spectroscopic methods offer versatile approaches for investigating protein structure, dynamics, and function.
Understanding the range of available spectroscopic techniques is crucial for addressing complex biological questions.

Outlook:

Continued advancements in spectroscopy will further enhance our ability to probe biological systems.
The integration of diverse spectroscopic approaches promises deeper insights into protein behavior.