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Updated: Mar 2, 2026

Fat Preference: A Novel Model of Eating Behavior in Rats
Published on: June 27, 2014
Study of the effects of nesfatin-1 on gastric function in obese rats
Guo-Tao Yang1, Hong-Ying Zhao1, Yu Kong1
1Guo-Tao Yang, Ai-Qin Dong, Department of Third Neurology, Cangzhou Central Hospital, Cangzhou Clinical Medical School of Hebei Medical University, Cangzhou 061001, Hebei Province, China.
Aim:
To investigate the effects of nesfatin-1 on gastric function in obese rats.
Methods:
The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes.
Results:
Body weight, body length, and Lee's index of the rats significantly increased in the high-fat diet-induced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. This in vivo experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.
Conclusion:
Through this research, we provide a theoretical basis for further studies on nesfatin-1, a potential anti-obesity drug.
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