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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Related Experiment Video

Updated: Mar 2, 2026

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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Editing plants for virus resistance using CRISPR-Cas.

J C Green, J S Hu

    Acta Virologica
    |May 20, 2017
    PubMed
    Summary
    This summary is machine-generated.

    Clustered regularly interspaced palindromic repeats-associated nuclease (CRISPR-Cas) systems offer precise plant trait editing for virus resistance. These systems show promise for controlling RNA and DNA viruses, including geminiviruses, in both transgenic and non-transgenic plants.

    Keywords:
    CRISPR-Cas; virus resistance; plant virus.

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    A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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    Area of Science:

    • Agricultural Science
    • Molecular Biology
    • Plant Pathology

    Background:

    • Classical plant breeding faces limitations in developing virus-resistant crops.
    • Gene silencing technologies have yielded inconsistent results for geminivirus resistance.
    • Prokaryote-derived CRISPR-Cas systems offer precise genome editing capabilities.

    Purpose of the Study:

    • To review recent advancements in using CRISPR-Cas systems for engineering plant virus resistance.
    • To discuss approaches for enhancing resistance in both transgenic and non-transgenic plants.
    • To evaluate the potential of CRISPR-Cas for controlling RNA and DNA viruses.

    Main Methods:

    • Review of scientific literature on CRISPR-Cas applications in plant virology.
    • Analysis of CRISPR-Cas systems, including FnCas9 and C2c2, for RNA editing.
    • Examination of CRISPR-Cas strategies targeting geminiviruses and ssDNA viruses.

    Main Results:

    • CRISPR-Cas systems enable precise trait insertion for improved plant breeding.
    • CRISPR-Cas engineered plants consistently show reduced geminivirus accumulation and increased resistance.
    • RNA-targeting CRISPR-Cas iterations (FnCas9, C2c2) are advantageous for combating RNA viruses.

    Conclusions:

    • CRISPR-Cas technology presents a powerful and precise tool for developing virus-resistant plants.
    • CRISPR-Cas offers a reliable strategy for controlling challenging plant viruses like geminiviruses and BBTV.
    • This technology holds significant potential for revolutionizing crop protection against viral diseases.