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Hemolysin production by Leptospira interrogans serovar canicola in a protein-free medium with hemin
E M Lane1, R F Ramaley, N G Miller
1Department of Medical Microbiology, University of Nebraska College of Medicine, Omaha 68105.
Abstract:
Leptospira interrogans serovar canicola strain moulton was grown to a high cell density in a protein-free medium. When hemin was added to this medium, hemolysin was produced. Hemolysin was not detected when other porphyrins or cytochrome C were substituted for hemin in the medium.