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Updated: Mar 2, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape
Kholod Alamoudi1, Patricia Martins1, Jonas G Croissant1
1Smart Hybrid Materials Laboratory, Advanced Membranes & Porous Materials Center, King Abdullah University of Science & Technology, Thuwal, Saudi Arabia.
Aim:
Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.
Methods:
A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.
Results:
The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.
Conclusion:
The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.
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