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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

Absolute quantification of DNA methylation using microfluidic chip-based digital PCR.

Zhenhua Wu¹, Yanan Bai¹, Zule Cheng¹

¹State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China; University of Chinese Academy of Sciences, Beijing 100039, China.

Biosensors & Bioelectronics

|May 20, 2017

Summary

This summary is machine-generated.

This study introduces a novel microfluidic chip-based digital PCR method for detecting DNA methylation. This sensitive and cost-effective technique shows promise for early cancer diagnosis by analyzing tumor suppressor gene methylation.

Keywords:

DNA Digital PCR Methylation Microfluidics

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Last Updated: Mar 2, 2026

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

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Published on: June 26, 2016

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Area of Science:

Epigenetics and Molecular Oncology
Biotechnology and Bioengineering

Background:

Aberrant DNA methylation, particularly promoter hypermethylation of tumor suppressor genes, is a hallmark of tumorigenesis.
Accurate detection of DNA methylation patterns is crucial for cancer diagnostics and understanding epigenetic alterations.

Purpose of the Study:

To develop and validate a novel, highly sensitive method for quantitative DNA methylation detection.
To assess the utility of microfluidic chip-based digital PCR for analyzing methylation in cancer-related genes.

Main Methods:

Utilized methylation-sensitive restriction enzyme HpaII to selectively cleave unmethylated DNA.
Employed microfluidic chip-based digital PCR for quantitative analysis of DNA methylation levels.
Validated the method by analyzing promoter methylation of PCDHGB6 and HOXA9 in lung adenocarcinoma tissues.

Main Results:

The developed method achieved a lower limit of detection of 0.52% for DNA methylation.
Quantitative methylation analysis showed consistency with conventional bisulfite pyrosequencing.
Successfully detected promoter methylation differences in tumor suppressor genes between cancerous and non-tumorous lung tissues.

Conclusions:

Microfluidic chip-based digital PCR offers a sensitive, cost-effective, and reliable approach for DNA methylation detection.
This method holds significant potential for the early diagnosis of epigenetics-related diseases, including cancer.
The technique provides a promising alternative for routine clinical diagnostics and epigenetic research.