Related Experiment Video
Updated: Mar 2, 2026
06:36
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
Published on: July 17, 2014
25.3K
Embryonic AP1 Transcription Factor Deficiency Causes a Collodion Baby-Like Phenotype.
Christina A Young1, Richard L Eckert2, Gautam Adhikary1
1Departments of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA.
The Journal of Investigative Dermatology
|May 21, 2017
Summary
Inhibiting AP1 transcription factors in embryonic skin disrupts filaggrin production and barrier function, causing ichthyosis-like symptoms and a collodion membrane. This highlights AP1
Area of Science:
- Dermatology and developmental biology
- Molecular genetics and skin barrier function
Background:
- AP1 transcription factors regulate epidermal gene expression, influencing keratinocyte proliferation and differentiation.
- Altered AP1 signaling is implicated in skin diseases, but its precise role in epidermal development and barrier formation remains unclear.
- Previous studies linked AP1 inhibition in adult epidermis to reduced filaggrin and an ichthyosis vulgaris-like phenotype.
Purpose of the Study:
- To investigate the role of AP1 transcription factors during embryonic epidermal development.
- To determine the impact of AP1 deficiency on filaggrin expression, skin barrier function, and associated phenotypes.
- To explore the potential contribution of AP1 deficiency and filaggrin loss to ichthyoses and collodion membrane formation.
Main Methods:
- Inhibition of AP1 transcription factor function during embryonic epidermal development in mice.
- Analysis of phenotypic changes, including filaggrin mRNA and protein levels, barrier function, and ultrastructural alterations.
- Assessment of dehydration susceptibility and newborn phenotypes, including collodion membrane formation.
Main Results:
- AP1 inhibition in embryonic epidermis led to reduced filaggrin mRNA and protein levels.
- Compromised skin barrier function, significant ultrastructural changes, and increased dehydration susceptibility were observed.
- AP1-deficient newborns exhibited a collodion membrane phenotype, distinct from the flaky tail mouse model of ichthyosis vulgaris.
Conclusions:
- AP1 transcription factor deficiency during development severely impacts epidermal differentiation and barrier formation.
- The observed phenotype, including filaggrin reduction and collodion membrane, suggests a role for AP1 in specific ichthyosis subtypes.
- Further research is needed to elucidate the combined roles of filaggrin loss and AP1 deficiency in ichthyoses and collodion membrane formation.
More Related Videos
Related Concept Videos
01:33Pleiotropy
43.6K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
43.6K
01:05Type IV Collagen of Basal Lamina
3.2K
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...
A type IV collagen molecule has six alpha chains which can...
3.2K