The effects of melamine with or without cyanuric acid on immune function in ovalbumin-sensitized mice

Melamine is commonly used in the chemical industry, and it has been found to exist on food processing equipment and utensils. Previous investigations suggested that melamine alone or its combination with cyanuric acid appears to be toxic to immune system in animals. The objective of this study is to investigate the potential effects of melamine with or without cyanuric acid on immune function in ovalbumin- sensitized mice. Our data indicated that melamine-related administration caused a significant decreasing in the content of IL-4 in mice in comparison to negative control group. Significant increasing in the content of histamine (HIS) was recorded in almost all treated groups. The co-administration of melamine and cyanuric acid with high dose (each at 16mgkg^-12d^-1) led to a significantly lower contents of IL-10, IL-16 and monocyte chemoattractant protein-1 (MCP-1) in mouse serum in comparison to negative control group. Moreover, our data indicated that the number of Th1 and Th2 cells, and the ratio of Th1/Th2 spleen lymphocytes were significantly changed after treatment. Also, our results demonstrated that the profiles of both CD40 and CD40L were significantly altered in spleen lymphocytes after treatment.