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Updated: Mar 2, 2026

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
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Buffer formulation affects the interaction between lysozyme and polymeric nanoparticles.

Martin Lundqvist1, Celia Cabaleiro-Lago1

  • 1Department of Biochemistry and Structural Biology, Lunds University, PO188, Sweden.

Journal of Colloid and Interface Science
|May 22, 2017
PubMed
Summary
This summary is machine-generated.

Buffer formulation impacts chicken egg lysozyme

Keywords:
BufferDynamicsInteractionIonic strengthNanoparticlesProteinZeta-potential

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Area of Science:

  • Biophysical Chemistry
  • Materials Science
  • Nanotechnology

Background:

  • Protein-nanoparticle interactions are crucial in various applications.
  • Understanding these interactions requires studying environmental factors like buffer composition.
  • Chicken egg lysozyme and carboxyl-modified polystyrene nanoparticles serve as a model system.

Purpose of the Study:

  • To investigate how buffer identity and ionic strength affect lysozyme adsorption onto nanoparticles.
  • To determine the impact of these buffer properties on protein conformation and nanoparticle stability.
  • To elucidate the kinetics and mechanisms of lysozyme-nanoparticle interactions under varying buffer conditions.

Main Methods:

  • Systematic study of buffer effects on protein-nanoparticle interactions.
  • Time-resolved fluorescence spectroscopy using a reporter molecule to monitor lysozyme adsorption.
  • Assessment of colloidal stability of nanoparticles.
  • Variations in buffer identity and ionic strength were employed.

Main Results:

  • Lysozyme adsorbs to nanoparticles under all tested buffer conditions.
  • Adsorption leads to changes in lysozyme's protein conformation.
  • Nanoparticle colloidal stability decreases upon lysozyme interaction.
  • Adsorption rate is primarily influenced by ionic strength, especially without background salt.
  • Increasing ionic strength slows down adsorption kinetics due to ion presence.

Conclusions:

  • Buffer ionic strength is a key factor governing lysozyme-nanoparticle adsorption dynamics.
  • Protein conformation changes and reduced nanoparticle stability are consequences of this interaction.
  • While buffer identity has minor effects, ionic strength significantly modulates the adsorption process.