Related Experiment Video
Updated: Mar 2, 2026

Experimental Metastasis Assay
Published on: August 24, 2010
Codonopsis lanceolata polysaccharide CLPS inhibits melanoma metastasis via regulating integrin signaling
Yanqiu Liu1, Xiangming Zou2, Guangren Sun3
1College of Forestry, Northeast Forestry University, Harbin 150040, China; College of Forestry, Beihua University, Jilin 132013, China.
Abstract:
β1 integrin-mediated migration plays a fundamental role in melanoma metastasis. Here, we evaluated the molecular mechanisms underlying the anti-metastatic capacity of a polysaccharide fraction (CLPS) from Codonopsis lanceolata. CLPS inhibited in vivo melanoma metastasis in a B16F10 pulmonary metastasis model, impaired β1 integrin-mediated B16F10 melanoma cell migration in vitro evaluated by Transwell assay, reduced affinity between β1 integrin and ligand protein GST-FNIII9-10. Also, CLPS attenuated the adhesion-dependent formation of focal adhesion and blocked β1 integrin/FAK/paxillin signaling axis. These findings illustrate a better understanding underlying the anti-metastatic activity of CLPS, indicating a potential treatment strategy for melanoma metastasis.
Related Concept Videos
Targeted Cancer Therapies
There are several types of targeted therapies against...
Activation of Integrins
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
Metastasis
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Integrins
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Cancer Cell Migration through Invadopodia
Selectins

