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PAQR3 inhibits the proliferation, migration and invasion in human glioma cells
Shi-Lei Tang1, Yuan-Lin Gao2, Wen-Zhong Hu1
1Department of Neurosurgery, Huaihe Hospital of Henan University, Kaifeng 475000, Henan Province, China.
Abstract:
Progestin and AdipoQ Receptor 3 (PAQR3), a member of the PAQR family, is down-regulated in several types of cancers and has been closely associated with tumor progression and development. However, little is known about the functions of PAQR3 in the tumorigenesis of human glioma. Therefore, in this report, we investigated the role of PAQR3 in human glioma. Our results showed that the expression of PAQR3 was significantly reduced in human glioma tissues and cell lines. PAQR3 overexpression inhibited the proliferation of glioma cells in vitro and attenuated tumor xenograft growth in vivo. In addition, PAQR3 overexpression suppressed the migration and invasion of glioma cells, as well as prevented the EMT process. Mechanistic studies demonstrated that PAQR3 overexpression significantly down-regulated the levels of phosphorylated PI3K and Akt in U251 cells. In conclusion, these results demonstrated that PAQR3 inhibited the proliferation, migration and invasion in glioma cells, at least in part, through the inactivation of PI3K/Akt signaling pathway. Therefore, PAQR3 may be a therapeutic target for the treatment of glioma.
Insights
Progestin and AdipoQ Receptor 3 (PAQR3) is reduced in glioma and inhibits tumor growth. PAQR3 inactivation of the PI3K/Akt pathway offers a potential therapeutic target for glioma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Progestin and AdipoQ Receptor 3 (PAQR3) is implicated in various cancers.
- PAQR3's role in human glioma tumorigenesis remains largely uncharacterized.
- PAQR3 expression is often reduced in cancerous tissues.
Purpose of the Study:
- To investigate the function of PAQR3 in human glioma.
- To determine the effect of PAQR3 on glioma cell proliferation, migration, and invasion.
- To elucidate the molecular mechanisms underlying PAQR3's action in glioma.
Main Methods:
- Analysis of PAQR3 expression in human glioma tissues and cell lines.
- In vitro studies on glioma cell proliferation, migration, and invasion upon PAQR3 overexpression.
- In vivo studies using tumor xenograft models.
- Western blot analysis to assess PI3K/Akt signaling pathway activation.
Main Results:
- PAQR3 expression was significantly decreased in human glioma tissues and cell lines.
- Overexpression of PAQR3 inhibited glioma cell proliferation in vitro and tumor growth in vivo.
- PAQR3 overexpression suppressed glioma cell migration and invasion, and prevented epithelial-mesenchymal transition (EMT).
- PAQR3 overexpression led to down-regulation of phosphorylated PI3K and Akt in U251 cells.
Conclusions:
- PAQR3 acts as a tumor suppressor in human glioma.
- PAQR3 inhibits glioma cell proliferation, migration, and invasion, partly via the PI3K/Akt signaling pathway.
- PAQR3 represents a potential therapeutic target for glioma treatment.
