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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
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Matrix MAPS-an intuitive software to acquire, analyze, and annotate light microscopy data for CLEM.

Martin Schorb1, Frank Sieckmann2

  • 1European Molecular Biology Laboratory, Heidelberg, Germany.

Methods in Cell Biology
|May 23, 2017
PubMed
Summary
This summary is machine-generated.

Matrix MAPS software simplifies correlative light and electron microscopy (CLEM) data acquisition. It enables automated imaging of defined regions at various temperatures and seamless transfer of coordinates to electron microscopes.

Keywords:
Automated microscopyCorrelative light and electron microscopyCryofluorescence microscopyImage analysis

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Area of Science:

  • Correlative light and electron microscopy (CLEM)
  • Microscopy data acquisition
  • Cryogenic and room temperature imaging

Background:

  • Correlative light and electron microscopy (CLEM) requires precise integration of imaging data from different modalities.
  • Efficiently acquiring light microscopy data that precisely corresponds to electron microscopy regions is challenging.

Purpose of the Study:

  • To introduce Matrix MAPS, an intuitive software interface for streamlining light microscopy data acquisition in CLEM workflows.
  • To enable automated, user-defined region selection and coordinate transfer for enhanced CLEM experiments.

Main Methods:

  • Graphical definition of acquisition regions on preview images.
  • Automated imaging of multiple, independent regions with customizable settings.
  • Selection of points and regions of interest within the light microscope interface.

Main Results:

  • Matrix MAPS provides an intuitive graphical user interface for defining light microscopy acquisition areas.
  • The software supports automated imaging of multiple regions with individualized settings.
  • Coordinates of selected points/regions of interest are directly transferable to electron microscopes.

Conclusions:

  • Matrix MAPS enhances the efficiency and precision of data acquisition for correlative light and electron microscopy.
  • The software facilitates seamless integration between light and electron microscopy by simplifying region selection and coordinate transfer.
  • Matrix MAPS is applicable to both room temperature and cryogenic CLEM experiments.