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Updated: Mar 2, 2026

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
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A new method for cryo-sectioning cell monolayers using a correlative workflow.

Androniki Kolovou1, Martin Schorb1, Abul Tarafder1

  • 1European Molecular Biology Laboratory, Heidelberg, Germany.

Methods in Cell Biology
|May 23, 2017
PubMed
Summary
This summary is machine-generated.

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This study introduces a new sample preparation method for cryo-electron microscopy (cryo-EM). This technique improves in situ structural analysis by enabling precise targeting of subcellular regions using correlative light and electron microscopy (CLEM).

Area of Science:

  • Structural Biology
  • Cell Biology
  • Microscopy

Background:

  • Cryo-electron microscopy (cryo-EM) offers near-atomic resolution of protein complexes.
  • In situ cryo-EM provides insights into molecular machines in their native cellular environment.
  • A key challenge for in situ cryo-EM is targeting specific subcellular locations.

Purpose of the Study:

  • To present a novel sample preparation technique for cryo-electron microscopy.
  • To enable precise targeting of subcellular regions for in situ structural analysis.
  • To bridge structural biology and cell biology using correlative light and electron microscopy (CLEM).

Main Methods:

  • Developed a sample preparation technique for cryo-sections of vitrified cell monolayers.
  • Ensured cryo-sections are oriented parallel to the fluorescence imaging plane.
Keywords:
CEMOVISCryo-sectionCryo-ultramicrotomyHigh-pressure freezingTargeted trimming

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  • Utilized correlative light and electron microscopy (CLEM) for targeted imaging.

    • Main Results:

    • The technique allows cryo-sections to be prepared in an orientation optimal for CLEM.
    • This facilitates the selection of specific cells within heterogeneous populations.
    • Enables the identification of specific subcellular regions on cryo-sections for detailed structural analysis.

    Conclusions:

    • The presented method enhances the capabilities of in situ cryo-EM by integrating CLEM.
    • It overcomes the challenge of focusing on specific subcellular regions.
    • This approach facilitates high-resolution structural studies of molecular machines in their native cellular context.