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Updated: Mar 2, 2026

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Knockdown of STMN1 enhances osteosarcoma cell chemosensitivity through inhibition of autophagy

Zili Wang1, Rongzhen He1, Hansong Xia1

  • 1Department of Orthopaedic Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.

Oncology Letters
|May 23, 2017
PubMed

Insights

Stathmin 1 (STMN1) promotes chemoresistance in osteosarcoma by activating autophagy. Reducing STMN1 enhances sensitivity to paclitaxel by inhibiting this process, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Chemoresistance significantly impacts osteosarcoma (OS) patient outcomes.
  • Mechanisms driving OS chemoresistance remain incompletely understood.

Purpose of the Study:

  • To investigate the role of stathmin 1 (STMN1) in paclitaxel-induced chemoresistance in OS.
  • To elucidate the underlying molecular mechanisms involving autophagy.

Main Methods:

  • Western blot analysis to assess STMN1 expression in OS cell lines and normal osteoblasts.
  • Paclitaxel treatment to induce chemoresistance and autophagy.
  • STMN1 knockdown experiments.
  • Mammalian target of rapamycin (mTOR) signaling pathway blockade.

Main Results:

  • STMN1 expression was significantly elevated in multiple OS cell lines compared to normal cells.
  • Paclitaxel treatment upregulated STMN1 and activated autophagy in U-2OS cells.
  • Knockdown of STMN1 reduced paclitaxel-induced autophagy and increased cancer cell sensitivity to paclitaxel.
  • mTOR pathway inhibition partially reversed the effect of STMN1 knockdown on autophagy.

Conclusions:

  • STMN1 promotes chemoresistance in osteosarcoma by enhancing paclitaxel-induced autophagy.
  • Targeting STMN1 can improve osteosarcoma chemosensitivity to paclitaxel via autophagy inhibition.
  • STMN1 represents a promising therapeutic target for overcoming chemoresistance in OS.
Keywords:
autophagychemoresistanceosteosarcomapaxilitaxelstathmin 1

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