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Related Concept Videos

Introduction to Hemostasis01:05

Introduction to Hemostasis

15.4K
Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
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Formation of the Platelet Plug01:22

Formation of the Platelet Plug

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The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
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Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

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Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
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Structure and Function of Platelets01:18

Structure and Function of Platelets

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The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
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Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

2.1K
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
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Coagulation01:09

Coagulation

11.3K
The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
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Related Experiment Video

Updated: Mar 2, 2026

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

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A microfluidic model of hemostasis sensitive to platelet function and coagulation.

R M Schoeman1, K Rana1, N Danes2

  • 1Chemical and Biological Engineering Department, Colorado School of Mines, Golden, CO.

Cellular and Molecular Bioengineering
|May 23, 2017
PubMed
Summary

This study introduces a novel microfluidic model for studying hemostasis and thrombus formation outside blood vessels. The model accurately simulates bleeding disorders like hemophilia A, aiding research into excessive blood loss.

Keywords:
biorheologybiotransportcoagulationplatelets

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Microfluidics in Assessing Platelet Function
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Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
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Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

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Related Experiment Videos

Last Updated: Mar 2, 2026

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

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Microfluidics in Assessing Platelet Function
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Microfluidics in Assessing Platelet Function

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Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

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Area of Science:

  • Biomedical Engineering
  • Hematology
  • Microfluidics

Background:

  • Hemostasis, the process of sealing vascular injuries, is crucial for arresting bleeding.
  • Existing models primarily focus on intravascular thrombus formation, with limited options for studying extravascular thrombus formation.
  • Understanding extravascular hemostasis is vital for managing bleeding disorders and trauma.

Purpose of the Study:

  • To develop and validate a microfluidic model simulating extravascular thrombus formation.
  • To investigate the roles of coagulation and platelet function in hemostasis using this model.
  • To provide a platform for studying coagulopathies and platelet dysfunction.

Main Methods:

  • A microfluidic device was engineered with vascular, vessel wall, and extravascular compartments.
  • Type I collagen and tissue factor were used to promote platelet adhesion and initiate coagulation.
  • Hemostatic challenges were simulated by inhibiting Factor VIII (hemophilia A model) and using a P2Y12 antagonist.

Main Results:

  • The model successfully formed a stable thrombus, arresting blood loss into the extravascular space within approximately 7.5 minutes.
  • Inhibition of Factor VIII led to unstable thrombi and failure to close the injury, mimicking hemophilia A.
  • P2Y12 antagonist treatment doubled the hemostasis closure time, indicating the impact of platelet function.

Conclusions:

  • The developed microfluidic model effectively replicates extravascular hemostasis and thrombus stabilization.
  • The model is sensitive to both coagulation and platelet function, making it suitable for studying bleeding disorders.
  • This platform offers a valuable tool for preclinical research on coagulopathies and platelet disorders.