Escherichia coli serotype K44: an acidic capsular polysaccharide containing two 2-acetamido-2-deoxyhexoses
G G Dutton1, D N Karunaratne, A V Lim
1Department of Chemistry, University of British Columbia, Vancouver, Canada.
Abstract:
The structure of the capsular polysaccharide from Escherichia coli O8:K44 (A):H- (K44 antigen) has been established using the techniques of methylation, beta-elimination, deamination, and Smith degradation. N.m.r. spectroscopy (13C and 1H) was used extensively to establish the nature of the anomeric linkages of the polysaccharide and of oligosaccharides derived through degradative procedures. The K antigen is comprised of repeating units of the linear tetrasaccharide shown. This acidic polysaccharide represents the first instance of an E. coli K antigen in this series (group A) that has been found to contain two different 2-acetamido-2-deoxyhexoses.
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