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Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease
Published on: February 3, 2012
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Capillary endothelia from two ADPKD patients are polyploidy
Sarmed H Kathem1,2, Wissam A AbouAlaiwi3, Xiaolin Zi2
1Department of Biomedical & Pharmaceutical Sciences, Chapman University, Irvine, CA.
Annals of Clinical Cytology and Pathology
|May 23, 2017
Summary
Autosomal dominant polycystic kidney disease (ADPKD) involves kidney cyst formation. Researchers found that not only kidney cells but also capillary endothelial cells in ADPKD patients exhibit polyploidy, suggesting it as a potential disease marker.
Area of Science:
- Nephrology
- Cell Biology
- Genetics
Background:
- Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder.
- Bilateral renal cyst formation is a primary characteristic of ADPKD.
- Previous studies identified polyploidy in cyst-lining epithelia of ADPKD kidneys.
Purpose of the Study:
- To investigate polyploidy in renal capillary endothelial cells of ADPKD patients.
- To determine if polyploidy is a broader cellular characteristic in ADPKD kidneys.
Main Methods:
- Flow cytometry analysis of kidney cells.
- Spectral karyotyping for chromosomal analysis.
- Examination of renal capillary endothelial cells from ADPKD patients.
Main Results:
- Endothelial cells from the renal capillary beds of ADPKD patients were found to be polyploid.
- Flow cytometry and spectral karyotyping confirmed the presence of polyploidy in these endothelial cells.
Conclusions:
- Polyploidy is present not only in cyst-lining epithelia but also in renal capillary endothelia in ADPKD.
- Polyploidy may serve as a potential cellular marker for diagnosing and understanding ADPKD.
