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Related Experiment Videos

The degranulation response in differentiated HL-60 cells.

L C Meagher1, T G Cotter

  • 1Department of Biology, St Patrick's College, Maynooth, Co. Kildare, Ireland.

Clinical and Experimental Immunology
|December 1, 1988
PubMed
Summary

HL-60 cell granulocytes show impaired degranulation, specifically reduced myeloperoxidase release, when stimulated with soluble aggregated IgG (SAIgG). This degranulation defect is specific to SAIgG and not observed with other common stimuli.

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Area of Science:

  • Cell Biology
  • Hematology
  • Immunology

Background:

  • HL-60 cells are a human promyelocytic leukemia cell line that can be differentiated into granulocyte-like cells.
  • Differentiated HL-60 cells mimic some functions of peripheral blood granulocytes.
  • The degranulation response is a critical function of granulocytes.

Purpose of the Study:

  • To investigate the degranulation response of HL-60 cell-derived granulocytes.
  • To compare the degranulation response to soluble aggregated IgG (SAIgG) with other stimuli.
  • To identify potential defects in HL-60 cell granulocyte degranulation.

Main Methods:

  • Differentiated HL-60 cells using dimethylsulfoxide (DMSO) or retinoic acid.
  • Stimulated differentiated HL-60 cells with various agents including SAIgG, fMet-Leu-Phe, A23187, and phorbol myristate acetate (PMA).

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  • Measured the release of granule enzymes like elastase and myeloperoxidase.

    • Main Results:

    • SAIgG primarily stimulated elastase release, with minimal myeloperoxidase release from HL-60 derived granulocytes.
    • This selective release contrasts with the response of peripheral blood granulocytes.
    • Degranulation responses to fMet-Leu-Phe, A23187, and PMA were relatively normal.
    • The defect was not attributed to enzyme inhibitors, stimulus binding, release kinetics, or enzyme compartmentalization.

    Conclusions:

    • HL-60 cell-derived granulocytes exhibit an impaired degranulation response specifically to SAIgG.
    • The degranulation mechanism for myeloperoxidase release is defective in response to SAIgG in these cells.
    • This suggests a specific pathway or component involved in IgG-mediated degranulation is altered in differentiated HL-60 cells.