Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same author

Clinical Outcomes and Applicability of Emergency Department Termination-of-Resuscitation Rules in Super-Elderly Patients with Out-of-Hospital Cardiac Arrest: A Multicenter Analysis.

Diagnostics (Basel, Switzerland)·2026
Same author

Morphology-Preserving Holotomography: Quantitative Analysis of 3D Organoid Dynamics.

IEEE transactions on medical imaging·2026
Same author

Label-free volumetric refractive-index imaging of fibrillar collagen architecture, assembly, and cell-associated remodeling.

bioRxiv : the preprint server for biology·2026
Same author

Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit.

bioRxiv : the preprint server for biology·2026
Same author

Cells Dynamically Adapt Their Nuclear Volumes and Proliferation Rates During Single to Multicellular Transitions.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: Mar 2, 2026

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

12.6K

Tomographic active optical trapping of arbitrarily shaped objects by exploiting 3D refractive index maps.

Kyoohyun Kim1,2, YongKeun Park1,2,3

  • 1Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.

Nature Communications
|May 23, 2017
PubMed
Summary

This study introduces a novel optical trapping method for precise 3D control of arbitrarily shaped particles. It enables real-time manipulation of non-spherical objects without prior geometric knowledge.

More Related Videos

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.6K
Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

23.0K

Related Experiment Videos

Last Updated: Mar 2, 2026

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

12.6K
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.6K
Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

23.0K

Area of Science:

  • Photonics
  • Soft Matter Physics
  • Biophysics

Background:

  • Optical trapping commonly manipulates spherical particles via predicted optical forces.
  • Controlling non-spherical particles in three dimensions (3D) with arbitrary orientations presents significant experimental and computational challenges.

Purpose of the Study:

  • To develop a method for real-time optical control of arbitrarily shaped particles.
  • To overcome the limitations of current optical trapping techniques for complex geometries.

Main Methods:

  • Combines wavefront shaping of a trapping beam with 3D refractive index distribution measurements.
  • Engineers the 3D light field distribution to create a 'light mould' for particle manipulation.

Main Results:

  • Achieved stable, real-time optical control of arbitrarily shaped particles.
  • Demonstrated manipulation of colloidal and biological samples with complex orientations and shapes.
  • Eliminated the need for a priori information on sample geometry.

Conclusions:

  • The developed light mould technique offers precise 3D manipulation of non-spherical particles.
  • This method has direct applications in biophotonics and soft matter physics research.
  • Enables new possibilities for assembling and controlling microscale objects.