A saliva molecular imprinted localized surface plasmon resonance biosensor for wine astringency estimation
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Updated: Mar 2, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Published on: November 23, 2015
J Rafaela L Guerreiro1, Natércia Teixeira2, Victor De Freitas2
1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark; BioMark Sensor Research-CINTESIS, Instituto Superior de Engenharia do Porto, Porto, Portugal; QUINOA-LAQV-REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Porto, Portugal.
Abstract:
Wine astringency was evaluated based on the interaction of two complex matrices (red wine and saliva) by combining localized surface plasmon resonance (LSPR) and molecular imprinted polymers (MIP) at gold nanodisks as an alternative to sensorial analysis. The main objective of the work was to simulate wine astringency inside the mouth by mimicking this biological system. The LSPR/MIP sensor provided a linear response for astringency expressed in pentagalloyl glucose (PGG) units in concentrations ranging from 1 to 140μmol/L. The sensor was also applied to wine samples correlating well with sensorial analysis obtained by a trained panel. The correlation of astringency and wine composition was also evaluated showing that anthocyanins may have an important role, not only for pigmentation but also in astringency.

