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Protein kinase C theta is dispensable for suppression mediated by CD25+CD4+ regulatory T cells

Kerstin Siegmund1, Nikolaus Thuille1, Katarzyna Wachowicz1

  • 1Department for Pharmacology and Genetics, Medical University Innsbruck, Innsbruck, Austria.

Plos One
|May 23, 2017
PubMed

Insights

Protein kinase C theta (PKCθ) is crucial for conventional T cells but not regulatory T (Treg) cell suppression. While PKCθ influences Treg cell differentiation in vivo, it is not required for their suppressive function.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Protein kinase C theta (PKCθ) is essential for conventional T cell activation.
  • The role of PKCθ in regulatory T (Treg) cell function remains unclear.

Purpose of the Study:

  • To investigate the role of PKCθ in Treg cell function and differentiation.
  • To determine if PKCθ is required for Treg-mediated suppression.

Main Methods:

  • Utilized siRNA and a selective pharmacological inhibitor (AEB071) to assess PKCθ function.
  • Examined Treg cell suppressive activity in vitro using PKCθ-deficient and wild-type mice.
  • Analyzed Foxp3 expression and cell percentages in lymphatic organs.

Main Results:

  • Murine Treg-mediated suppression in vitro was independent of PKCθ.
  • Treg cells from PKCθ-deficient mice exhibited normal suppressive activity in vitro.
  • A reduced percentage of Foxp3+CD25+CD4+ T cells was observed in lymphatic organs of PKCθ-deficient mice, suggesting a role in Treg differentiation in vivo.

Conclusions:

  • PKCθ is dispensable for Treg-mediated suppression.
  • PKCθ plays a role in the in vivo differentiation of Treg cells.

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