Effect of high-speed rolling on herpes simplex virus detection and replication

Area of Science:

• Virology
• Cell Culture Technology

Background:

• Herpes simplex virus (HSV) is a common human pathogen.
• Rapid and efficient detection of HSV is crucial for timely clinical management.

Purpose of the Study:

• To investigate the impact of high-speed rolling on herpes simplex virus replication and detection.
• To determine optimal rolling speeds for enhanced viral yield.

Main Methods:

• Inoculated cell cultures were subjected to different rolling speeds (2, 96, and 383 rpm) or kept stationary.
• Viral replication was assessed by quantifying foci formation and viral yield at 24 hours.
• Cytopathic effects were monitored up to 96 hours.

Main Results:

• Cultures rolled at 96 rpm demonstrated a 6.8-fold increase in foci compared to stationary cultures (P < 0.01).
• Viral yields were significantly higher in cultures rolled at 96 rpm (7.3-fold increase) and 2 rpm (2.9-fold increase) versus stationary controls.
• A higher percentage of cultures rolled at 96 rpm (89%) exhibited cytopathic effects by 96 hours compared to 2 rpm (45%) and stationary (5%) groups (P < 0.01).
• Maximum viral yields were achieved 2 days earlier in cultures rolled at 96 rpm.

Conclusions:

• High-speed rolling, particularly at 96 rpm, significantly enhances herpes simplex virus replication and accelerates the appearance of cytopathic effects.
• The rolling technique shows promise for improving the speed and sensitivity of HSV detection in clinical laboratories.
• Implementing rolling cultures could lead to more rapid diagnosis and treatment initiation for HSV infections.