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Updated: Mar 2, 2026

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
Published on: January 26, 2019
Immunomodulatory function of whole human umbilical cord derived mesenchymal stem cells
Hao Zhang1, Yanling Tao2, Haihui Liu3
1Department of Hematology, Affiliated Hospital of Jining Medical University, No. 79, Guhuai Road, Jining, Shandong 272000, China; Hematopoietic Stem Cell Transplantation, Affiliated Hospital to Academy of Military Medical Sciences, No. 8, East Street, Fengtai District, Beijing 100071, China.
Abstract:
Bone marrow derived mesenchymal stem cells (MSCs) play a critical role in immune modulation. However, immunomodulatory function of whole human umbilical cord derived mesenchymal stem cells (UC-MSCs) remains unclear. In this study, UC-MSCs were separated from whole umbilical cord using a single enzyme digestion. UC-MSCs (CD73+, CD90+, CD105+, and CD34-, CD45-, HLA-DR-) were differentiated into adipocytes, osteocytes and chondrocytes in vitro under specific stimulatory environments. UC-MSCs suppressed umbilical cord blood lymphocyte proliferation stimulated by mitogen, and ELISA showed that the secretion of INF-γ was downregulated, and the secretion of IL-4 was upregulated, with CD8+ T cells markedly decreased and CD4+ T cells changed lightly. Moreover, the infusion of UC-MSCs in recipient mice transplanted with donor bone marrow cells ameliorated acute graft-versus host disease (aGVHD) and extended survival. In conclusion, UC-MSCs might negatively modulate immunoreactions, and have application potential in the treatment of aGVHD caused by allogeneic stem cells transplantation.
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